I may be the outsider but I will place my scientific credibility on the line and agree with FAO and strongly disagree with AA and RZ. UV will degrade many nutrients especially B-vitamins. UV radiation can penetrate PET plastic. Phenol Red is a "strange bird". It can have estrogenic effects. It can also have pro- and anti- oxidant effects. It can also serve as a sink for UV irradiation and transmute (chemists in the audience please show us the mechanism) into reactive species. It is also particularly true that styrene and poypropylene plastics and/or their plasticizers are extremely reactive with UV irradiation. In my hands, no cells ever see phenol red or UV irraditated materials. I do work primarily with primary cells so my bias may not app;y to cancer cell lines.
Left to mean, i will like to ask why you want to put your DMEM medium under the UV light, for sterility or what?
prepare your media under aseptic conditions and control a sample of your prepared media for sterility. Prolonged exposure of prepared media to UV light, will degrade many nutrients and ingredients and should be avoided. i think this will help
I dont think it will do anything. Since in culture hood UV is mild and it can not penetrate the plastic or glass surface.
I agree with Aftab Alam. We usually pour the DMEM soultion with pipette and put it into plastic bottles. The UV light therefore can not hamper the solution
I may be the outsider but I will place my scientific credibility on the line and agree with FAO and strongly disagree with AA and RZ. UV will degrade many nutrients especially B-vitamins. UV radiation can penetrate PET plastic. Phenol Red is a "strange bird". It can have estrogenic effects. It can also have pro- and anti- oxidant effects. It can also serve as a sink for UV irradiation and transmute (chemists in the audience please show us the mechanism) into reactive species. It is also particularly true that styrene and poypropylene plastics and/or their plasticizers are extremely reactive with UV irradiation. In my hands, no cells ever see phenol red or UV irraditated materials. I do work primarily with primary cells so my bias may not app;y to cancer cell lines.
I would first like to thank you for your answers. I was studying an indirect cytotoxicity on polymers. It was necessary to keep them overnight in the cabin (With DMEM). Then i closed the cabin and made UV sterilization. I wanted to know why the cells die (In MTT test).
Now, i am trying again and i will learn the certain answer two days later.
Michael B LoMonaco if yo disagree with me please read this article "Treating cell culture media with UV irradiation against adventitious agents: Minimal impact on CHO performance". Sevgi i think it will help you.
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