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Questions related from Fadaka Adewale, Oluwaseun
I recently performed Bulk RNA sequencing on my samples (I have three conditions). This is my first time doing this. I would love to know what is expected of the sequencing data. In addition, are...
04 April 2024 1,652 0 View
Dear scholars, I am currently working on a project involving in silico analysis of some active constituents identified by HPLC. So far, I have done molecular docking using the extra precision (XP)...
22 August 2021 2,600 3 View
Dear experts, I have some challenges using HMMER therefore, I downloaded a user-friendly UGene software. What are the right classifications and the simplified way of using HMMER in peptide...
07 March 2020 2,755 1 View
the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...
20 June 2019 2,923 2 View
Dear all, Please i need to know the perfect procedure to separate lignin from other plant products. I need a procedure that will effectively remove lignin and to have as much cellulose as possible...
06 September 2018 8,274 4 View
Hello all, I am interested in prioritizing my genes (84) and the corresponding miRNAs (6) so I want to predict their expression levels, and subject them to prognostic analysis but most of these...
13 August 2018 1,098 0 View
Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...
30 April 2018 6,213 4 View
I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks
30 April 2018 1,628 2 View
microRNA as a short 18-22 nucleotide sequence has been implicated in gene expression regulation through its seed region base pairing to mRNA which degrades or prevent the binding to the promoter...
04 April 2017 6,604 1 View
aside the fact that there is high glucose concentration in the blood of a diabetic individual, is there a different in maybe rate of sedimentation or colour or what other difference can be revealed ?
23 January 2017 7,703 5 View
One miRNA could have several targets, but these targets may not all be regulated by the miRNA in every circumstance. For standard utilization of miRNAs as biological function and to prevent the...
21 April 2016 1,717 2 View
Can anyone suggest the easiest way to blast a given sequence aside the idtdna.com. I really need to learn more about blasting. Thanks
04 January 2016 7,601 3 View
I just wonder even after following al the protocols and preparing the stacking gel, it will only form a semi-solid gel. And refuse to solidify like the loading gel why?
01 September 2015 9,659 2 View
I just got this urgent work to do and i am sure researchers on research gate are far more capable to assist than anywhere else. I need protocols and procedures be it in word or pdf on the...
26 August 2015 8,635 2 View
I was to carry out a research in our lab and the first step is to purify protein extracted from the sample and check bands using SDS-PAGE. The procedure used for the resolving gel are as follows;...
04 August 2015 5,560 4 View
I am sure if the binding site of a microRNA can be implicated on a gene or mRNA there one can conclude about the mechanism of action and in a cancer case, its parthenogenesis. Is the binding on...
01 January 1970 1,403 5 View
