Hi All. If I UV treat culture supernatants to inactivate virus, will it affect/alter/destroy epitopes that I may want to analyze by Luminex or ELISA? Cheers.
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.
23 July 2024 4,800 1 View
I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...
27 June 2024 9,627 1 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....
17 June 2024 8,956 5 View
can someone use quantum mechanics to explain the intuition behind this phenomenon?
07 June 2024 9,584 2 View
Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...
07 June 2024 8,723 2 View
to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?
06 June 2024 5,095 1 View
Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...
31 May 2024 4,613 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View