Hi All. If I UV treat culture supernatants to inactivate virus, will it affect/alter/destroy epitopes that I may want to analyze by Luminex or ELISA? Cheers.
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...
22 February 2021 2,072 3 View
Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...
21 February 2021 6,389 3 View
Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...
17 February 2021 1,012 3 View
Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...
15 February 2021 7,703 4 View
This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...
15 February 2021 1,975 15 View
Hi Can anyone please suggest me how can I study the parameters of solar cells made of graphene oxide and any other organic material composite using SILVACO. I would also like to know how I can add...
15 February 2021 5,795 3 View
Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...
13 February 2021 3,517 2 View
Hi Can anyone please help me know the XRD details of reduced graphene oxide, Nitrogen doped reduced graphene oxide, Sulphur doped reduced graphene oxide, Nitrogen and Sulphur co-doped reduced...
08 February 2021 3,033 3 View
This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....
08 February 2021 8,321 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...
28 February 2021 2,723 4 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View