Michael Davis

6 Questions 52 Answers 0 Followers

Questions related from Michael Davis

Does UV inactivation of virus destroy epitopes within the sample?

Hi All. If I UV treat culture supernatants to inactivate virus, will it affect/alter/destroy epitopes that I may want to analyze by Luminex or ELISA? Cheers.

02 June 2020 5,583 0 View

How do you account for dying animals when graphing average tumor volume over time?

As the title suggests, we are testing treatment on tumor volume over time. However, if tumors reach 2000 mm3, we must sacrifice the animal. Obviously this will depress the average tumor volume...

02 October 2019 4,591 0 View

Can I use crystal structures of related proteins to generate a model for my protein of interest?

I have use Phyre2 to generate several partial structures in pdb format of my protein of interest (POI). This family of proteins dimerizes. I'd like to generate a model of the full dimerized POI....

16 August 2019 834 3 View

How would you positively identify a calpain cleavage site?

I would like to identify a calpain cut site in a 190 kDa protein. My plan is to express a c-terminally tagged protein, purify it by IP, and then conduct an on-bead digestion. Once digested, the...

26 February 2019 4,097 1 View

What is the best way to remove extracellular bacteria from Salmonella infected BMDMs?

I am infecting BMDMs with GFP-expressing Salmonella. I need to remove as many extracellular bacteria as possible. In a trial experiment, we rinsed the BMDMs after 30 minutes of infection and...

20 September 2018 2,014 4 View

Co-transfection: Looking for paper demonstrating that multiple plasmids are taken up by the same cells.

As the title indicates, I am looking for an old paper I once saw demonstrating that co-transection results in all plasmids being taken up by transfected cells. Any help would be appreciated.

01 January 1970 5,404 1 View