I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry data I found GFAP expression in contralateral site similar to the ipsilateral site, even after 7 days. But my data is about protein. So, based on other reports indicating increase in mRNA level in contralateral site, is it appropriate to come on to a conclusion of increase in protein expression too? Does increase in mRNA expression of particular gene justify increase in protein expression as well??
Hi Amisha,
You have two different questions, and I'll try to respond to both.
--> An increased expression of a protein is not necessary the consequence of an increase in mRNA expression, but seems quite likely conceivable.
--> GFAP increase in immunohistochemistry data, even in controlateral side to a lesion, means the presence of a glial scar (astrocytes), so the presence of a lesion. This controlateral lesion could be secondary to the lesion in ispilateral side, by apoptosis or neuroinflammation (which occur days until months after the insult) of the white matter tracts in the corpus calloseum (for example).
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