Amisha Parmar

12 Questions 5 Answers 0 Followers

Questions related from Amisha Parmar

How effective bioluminescence (IVIS Imaging) measurement is considered for quantitative purpose?

Hi, I consider "Total flux (photons/s)" for data analysis. However, when I compare the BLI data with tumor volume data (measured using digital vernier caliper), it mostly doesn't show...

10 October 2019 9,728 2 View

For triple negative breast cancer, which model is more suitable?

Hi all, While looking for TNBC models, I came across two models: 1. Orthotopical Intraductal Injection 2. Orthotopical mammary fat pad injection Which one is more suitable, if I want to see...

09 May 2019 5,651 3 View

What is the average weight of hypothalamus in Rat?

Hi all, please help me with this question. Thanks a lot in advance!

19 April 2019 4,598 3 View

Does unilateral entorhinal cortex lesion lead to changes in contralateral hippocampi??

I came across some literature showing the evidences of equally altered gene expression of some markers in contralateral site in stroke and EC Lesion. In my experiment, from imuunohistochemistry...

13 December 2017 8,204 1 View

What is the appropriate method for quantification in Immunohistochemistry when there is a decrease in cell density when compared to sham group?

I am working on neurodegeneration animal models, where i see that nerve cell count is CA1 region decreases drastically as compared to Sham. When proteins stain cell bodies, I quantify them in...

12 December 2017 2,588 0 View

Can anyone provide the protocol for Acridine orange/Ethidium bromide staining to visualize apoptosis?

Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...

19 September 2017 7,838 5 View

What things should be taken care of, while performing immunohistochemistry of phospho proteins?

I am doing IHC for normal proteins, but there must be a different protocol to treat phospho proteins. Please, enlighten me with that. Thanks!

03 August 2017 7,614 2 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

02 August 2017 10,094 2 View

What are the markers I can study for apoptosis in stroke model?

I have the idea about PARP-1 and Caspases which are usually taken as apoptotic markers. But still if anyone can enlighten me with some more idea. It would be great if anyone can share some...

29 June 2017 8,080 2 View

Which vendor is recommended for the purchase of IHC kit?

I am aware of the Novolink polymer detection system kit, Leica Biosystems. But I would like to know about other vendors for the same kit that might be better than this one. Thanks,

16 May 2017 5,244 2 View

What can be the ideal thickness of the tissue section for IHC?

Hi, I want to do immunohistochemistry of GFAP in brain tissue (coronal sections). And this the first time I am doing IHC of mouse brain tissue. Normally for histopathology we take 5 micron...

11 May 2017 10,252 11 View

Which dealer would be the best to buy APTT Reagent for Heparin sodium assay?

Hi, In our laboratory, We are planning to establish Heparin sodium assay as per IP as well as BP. As per the method mentioned in BP, we require APTT (Activated Partial Thromboplastin Time)...

25 January 2016 8,342 1 View