Amisha Parmar

6 Questions 3 Answers 0 Followers

Questions related from Amisha Parmar

How effective bioluminescence (IVIS Imaging) measurement is considered for quantitative purpose?

Hi, I consider "Total flux (photons/s)" for data analysis. However, when I compare the BLI data with tumor volume data (measured using digital vernier caliper), it mostly doesn't show...

10 October 2019 9,643 2 View

What is the average weight of hypothalamus in Rat?

Hi all, please help me with this question. Thanks a lot in advance!

19 April 2019 4,455 3 View

Can anyone provide the protocol for Acridine orange/Ethidium bromide staining to visualize apoptosis?

Hi everyone, Here I am working on neurodegeneration model and I am in a need to visualize apoptosis in early as well as late phase. I am aware of TUNEL assay, but I am more interested in...

19 September 2017 7,790 5 View

What things should be taken care of, while performing immunohistochemistry of phospho proteins?

I am doing IHC for normal proteins, but there must be a different protocol to treat phospho proteins. Please, enlighten me with that. Thanks!

03 August 2017 7,521 2 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

02 August 2017 9,992 2 View

What can be the ideal thickness of the tissue section for IHC?

Hi, I want to do immunohistochemistry of GFAP in brain tissue (coronal sections). And this the first time I am doing IHC of mouse brain tissue. Normally for histopathology we take 5 micron...

11 May 2017 10,020 11 View