Yes, the type of plasmid can affect protoplast transfection efficiency. Different plasmid backbones with the same vector size may have varying replication origins, promoters, and selectable markers, influencing gene expression and copy numbers.
Optimizing the plasmid design is crucial to achieve efficient protoplast transfection and desired transgene expression levels.
Some backbones even have additional elements that aid with transfection efficiency like DNA nuclear targeting sequences or a filamentous phage sso which has residual activity in the plant host.
Robert Adolf Brinzer Thanks. I don't know about any of these elements in my two vectors as I randomly choose, vectors are almost the same size as the reporter gene.
I would consider checking if they have a f1/fd/m13 ori annotated.
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