How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

How to measure the bonding strength of transfered graphene on PDMS substrate?

Chemical vapor deposition (CVD) was used to grow graphene on copper (Cu ) substrate, then the single layer graphene was transfered onto a PDMS substrate. I want to measure the bonding strength of...

17 October 2020 3,567 3 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

What form of Mg will be selected during the purification of prokaryotic expression protein?

Hello everyone Recently I wanted to purify several proteins by prokaryotic expression, I read some related literature and found that Mg is needed in the protein purification buffer. I am a little...

02 May 2020 931 3 View

Free acid and its salt, can HPLC-PDA-MS tell its difference?

I have a free acid R-COOH and its salt R-COOM. Both are insoluble in water but soluble in methanol. When their methanol solutions are injected into a HPLC-PDA-MS system, are they going to have the...

06 March 2020 4,952 4 View

Sodium chloride solution saturated vapor pressure at sub zero temperature?

Can anyone suggest where to find the data or any equations related to the salt solution vapor pressure at sub-zero temperature? Many thanks!

24 December 2019 7,741 2 View

[Systematic Reviews] What to do if publication bias exist?

When doing the systematic reviews, if you I confirmed a publication bias during meta-analysis, my questions is what to do next? I though to add discussion about the publication bias, but I want...

17 November 2019 5,634 4 View

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26 October 2019 7,914 3 View

How does acridine orange works to recognize apoptosis, autophagy or lysosomal stability?

I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...

08 August 2019 9,319 5 View

If two shRNA linked together, it works or not?

03 August 2019 299 3 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

SiRNA transfection efficency and results interpretation ?

i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...

11 February 2021 8,145 3 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

20 January 2021 8,221 2 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

Why T98g cells were dead after plamid transfection?

I constructed a plasmid, and extracted more than 500ug plasmid. I transfected it with four different reagent treatments, including two kind of nanoparticles ,pei and lipofectamin 3000. as a sample...

30 December 2020 7,412 0 View

What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

25 December 2020 8,668 3 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is there an increase in cell size after transfection?

We transfected AML cell line KG1 with the reagent lipofectamine LTX 300, and noticed some cells with the same shape, but twice bigger than usual. Maybe someone had also such experience, and could...

01 November 2020 4,519 3 View