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Hi all, I wonder the reason for deleting C-term lysine during antibody engineering? Thanks
19 September 2023 3,107 5 View
Hi all, Sometimes, transient expression using PEI system needs filler DNA or Boost DNA, but I can not find any paper describing transient expression using lipofectamine system needs filler DNA or...
24 February 2023 4,705 1 View
Firsty, I found that fucosylation level of anti-A protein antibody is significantly lower than anti-B protein antibody with ELISA using lectin. (Coating: A or B protein, Blocking, Sera,...
29 December 2015 7,761 1 View
Hello I have a really confusing question. I wanna count the cell of liquid based cytology(LBC). But the cell morphology is different between normal LBC and cancer LBC. And normally, cancer LBC...
12 February 2015 2,341 4 View
For cervical cancer, it is infected by human papillomavirus that contain two structural proteins and other oncoproteins. But there are about 20% of patients are anti-HPV16 E7 negative. However...
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Some antibodies are just used for western blotting, and some antibodies are just used for ELISA. So what is the difference? I producted serum antibody with Virus particle which has several major...
05 September 2013 7,835 8 View
I am trying to immunize a mouse with proteins but the proteins are proving hard to purify. I want use SDS-PAGE gel to seperate the proteins and immunize the protein with gel. How can I do this?...
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