We know that transfection forcing us in the HaCaT cell lines. I use different transfection agent PEI or Lipo. But I wonder if I use Basal HaCaT cell line ı could change transfection effiency?
Thanks Regards.
Dear Umit, try to eliminate the factors that caused the transfection to fail. In particular, it is recommended to use cells with 30 or fewer passages. The viability of the cells before transfection should be 90% and above, and there should be sufficient time for recovery after passage. Another thing that the logarithmic phase of the cells is also important at the time of transfection. Actively dividing cells are also more vulnerable to transfection agents such as toxic substances than the cells in the quiescent phase. The quality and the quantity of transfection nucleic acids and serum-lipid-antibiotic containing of the medium can all play a central role in your process efficacy. For example, an increase in the amount of antibiotics entering the cell with transfection will cause toxicity.
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