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We collected a specimen from Turkey. I think it is parasitic, do you have any idea for its family and genus?

05 November 2020 8,513 3 View

Which materials can be used as mordant for better adherence of plant derived stain to be used in tissue sections?

I'm working on tissue stains alternative to Hematoxylene in pathology laboratory routine. Which mordant can I use for better adherence?

10 June 2020 1,692 3 View

Can sleep problems in patients with Parkinson's disease be about serotonin?

Sleep problems can be seen in patients with Parkinson's disease. Can it be about serotonin? In mice, ablation of the raphe and no production serotonin increases wakefulness and impairs the...

05 March 2020 843 3 View

Does transfection efficiency increase in the basal transformed HaCaT (Human Keratinocyte) cell line?

17 October 2019 2,484 1 View

Could you please help me that how I can select a common seed at each run PROCESS MACRO?

05 January 2018 5,510 2 View

Can anyone suggest a succesful technique for the preparation of boron nanoparticles for biological purposes?

I am planning to synthesize boron nanoparticles and make surface modifications of them for biological applications. In literature, there are several methods but it seems that agglomeration of...

18 February 2016 9,474 4 View

Pethidine and norpethidine measurement in urine sample

especially with GC/MS analyse and what is the cut-off levels of pethidine and norpethidine in urine

16 February 2012 3,140 1 View

In may TD-DFT calculation result with GAUSSIAN 09w , oscillator strength value is too low, what is the probable causes, TD-DFT,B3LYP,G316(d,p)

In may TD-DFT calculation result with GAUSSIAN 09w , oscillator strength value is too low or 0, what is the probable causes, TD-DFT,B3LYP,G316(d,p)

01 January 1970 5,567 3 View

Schizophrenia and Medication

If we give someone medication who has a genetic predisposition for schizophrenia before its symptoms occur, can it be successful to abolish symptoms completely? Please share with me if you know...

01 January 1970 891 11 View

Why antidepressants help only some people?

What are the factors that predispose patients to treatment-resistant depression? What are the advances that predict antidepressant treatment response for depression? Two articles about...

01 January 1970 2,646 22 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View