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We collected a specimen from Turkey. I think it is parasitic, do you have any idea for its family and genus?

05 November 2020 8,513 3 View

NMR metabolomics identification software?

Hi everybody, Does anybody know an alternative way to identify and quantify the 1D-H NMR spectrum for metabolomics studies other than Chenomx? We have no budget for Chenomx and I need a freeware...

29 August 2020 2,877 3 View

GC-MS Column for Metabolomics Studies?

28 June 2020 8,404 3 View

Can sleep problems in patients with Parkinson's disease be about serotonin?

Sleep problems can be seen in patients with Parkinson's disease. Can it be about serotonin? In mice, ablation of the raphe and no production serotonin increases wakefulness and impairs the...

05 March 2020 843 3 View

Does transfection efficiency increase in the basal transformed HaCaT (Human Keratinocyte) cell line?

17 October 2019 2,484 1 View

Could you please help me that how I can select a common seed at each run PROCESS MACRO?

05 January 2018 5,510 2 View

Can anyone suggest a succesful technique for the preparation of boron nanoparticles for biological purposes?

I am planning to synthesize boron nanoparticles and make surface modifications of them for biological applications. In literature, there are several methods but it seems that agglomeration of...

18 February 2016 9,474 4 View

Can anyone help me with SDS Page Gel Electrophoresis destaining?

05 March 2015 7,503 7 View

Pethidine and norpethidine measurement in urine sample

especially with GC/MS analyse and what is the cut-off levels of pethidine and norpethidine in urine

16 February 2012 3,140 1 View

In may TD-DFT calculation result with GAUSSIAN 09w , oscillator strength value is too low, what is the probable causes, TD-DFT,B3LYP,G316(d,p)

In may TD-DFT calculation result with GAUSSIAN 09w , oscillator strength value is too low or 0, what is the probable causes, TD-DFT,B3LYP,G316(d,p)

01 January 1970 5,567 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View