Ümit Uzun

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Questions related from Ümit Uzun

Is it important to Cas9 activity 1 or 2 mismatch for designed gRNA?

Hi everyone, We designed gRNA with a maximum lenght of 20 nucleotide and I check my gRNA with Human Genom Browser/Blat web tools no matches found. However I use CCTop web tools (...

23 January 2020 2,369 0 View

Does transfection efficiency increase in the basal transformed HaCaT (Human Keratinocyte) cell line?

We know that transfection forcing us in the HaCaT cell lines. I use different transfection agent PEI or Lipo. But I wonder if I use Basal HaCaT cell line ı could change transfection...

18 October 2019 2,573 1 View