Many protocols suggest different speeds and time duration of shaking , on an orbital shaker, but they don't mention if it has to be done inside the incubator, or if it can be done outside, on a table top, or maybe inside a LAF hood.
Attila Lehotzky
It can be done on shaker, in the lab, at room temperature, but in closed flasks (vented cap should be covered by parafilm, for example - you need flask for this protocol) . You need a circular shaker, the rpm depends mostly the radius (optimally 1 cm around). It was suggested that the speed would be just lower than feeding astocytes detach. Similar method exists to isolate oligo precursors - do not mix the two protocols.
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