I am trying to cultivate Capan-1 and Capan-2 cell lines with RPMI 1640 medium supplemented with 20% FBS and antibiotics. But they are growing at a very slow rate. Is it normal? What is the doubling time of these cell lines? And how about other pancreatic cancer cell lines, like PANC-1, MIA PaCa-2, AcPC-1 and BxPC-3?
Thanks in advance.
For each of these cell lines, you can find the answer in the relevant Cellosaurus entries.
For example for CAPAN-1:
https://web.expasy.org/cellosaurus/CVCL_0237
Doubling time: ~50-100 hours (DSMZ); ~49 hours (PBCF).
For CAPAN-2:
https://web.expasy.org/cellosaurus/CVCL_0026
Doubling time: 96 hours (PubMed=3019537); ~50-70 hours (DSMZ).
Currently there are ~4'500 cell lines with such information in the Cellosaurus. So its far from complete, mostly because this type of information is unreported by authors who establish cell lines and by cell collections that distribute them. Fortunatly some do report this information as it is the case for DSMZ or JCRB.
Best regards
Amos Bairoch
Check all your cell lines for mycoplasma contamination. You can do this using commercial assays, some of which are based on PCR. Unusually slow growing cancer cell lines may be a sign of mycoplasma contamination.
Thanks for the answers, Mr. Amos Bairoch and Mr. Adam Karpf. The cell lines are free from mycoplasma contamination. Indeed the Cellosaurus entries shows a high doubling rate for these cell lines. I think I will try to grow them in flasks/dishes coated with Collagen type I and see what happens. Thanks once again.
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