9 Questions 8 Answers 0 Followers
Questions related from Alan Jhones Barbosa de Assis
Hello there. I will try to transient transfect the U-2 OS cell line (an osteosarcoma cell line) with miRNA mimics using Lipofectamine RNAiMAX in a 6-well plate format. Does anyone have any...
11 November 2019 3,044 3 View
05 November 2019 1,598 3 View
Hello. I want to transient tranfect PANC-1 cells with a microRNA mimic with Lipofectamine2000 reagent in 6-well plates at a final culture volume of 2 mL/well. So, after platting cells and removing...
15 August 2019 7,432 3 View
08 August 2019 8,880 3 View
I have downloaded the miRNA-seq data for pancreatic cancer from TCGA. Now, I would like to see the expression of a particular miRNA across all the samples - or at least to classify samples into...
10 July 2019 5,040 1 View
07 July 2019 1,787 1 View
Hello. I'm interested in transfection of Panc-1, MIA PaCa-2 and Capan-2 cell lines with microRNAs (transient transfection). I know these pancreatic cancer cell lines are difficult to transfect....
24 November 2018 6,332 2 View
11 November 2018 1,886 2 View
I am trying to cultivate Capan-1 and Capan-2 cell lines with RPMI 1640 medium supplemented with 20% FBS and antibiotics. But they are growing at a very slow rate. Is it normal? What is the...
10 October 2018 2,081 3 View
