Does anyone have a protocol for transfection of U-2 OS cell line?

Hello there. I will try to transient transfect the U-2 OS cell line (an osteosarcoma cell line) with miRNA mimics using Lipofectamine RNAiMAX in a 6-well plate format. Does anyone have any...

10 November 2019 2,985 3 View

Transient transfection medium in wells must be totally serum free?

Hello. I want to transient tranfect PANC-1 cells with a microRNA mimic with Lipofectamine2000 reagent in 6-well plates at a final culture volume of 2 mL/well. So, after platting cells and removing...

14 August 2019 7,364 3 View

Transient transfection medium in wells must be totally serum free?

Hello. I want to transient tranfect PANC-1 cells with a microRNA mimic with Lipofectamine2000 reagent in 6-well plates at a final culture volume of 2 mL/well. So, after platting cells and removing...

07 August 2019 8,808 3 View

How I can process miRNA-seq TCGA data to quantificate miRNA expression?

I have downloaded the miRNA-seq data for pancreatic cancer from TCGA. Now, I would like to see the expression of a particular miRNA across all the samples - or at least to classify samples into...

06 July 2019 1,732 1 View

Does anyone have a protocol for microRNA transfection of pancreatic cancer cell lines with Lipofectamine?

Hello. I'm interested in transfection of Panc-1, MIA PaCa-2 and Capan-2 cell lines with microRNAs (transient transfection). I know these pancreatic cancer cell lines are difficult to transfect....

10 November 2018 1,842 2 View

Does the pancreatic cancer cell lines Capan-1 and Capan-2 grows slowly?

I am trying to cultivate Capan-1 and Capan-2 cell lines with RPMI 1640 medium supplemented with 20% FBS and antibiotics. But they are growing at a very slow rate. Is it normal? What is the...

09 October 2018 2,026 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View