How to make ppb level concentration ?

Hi, I've N2 gas cylinder and 100 ppm NH3 gas cylinder. With these two cylinders, I'd like to make a ppb level (1- 100) concentration. Any idea how to do that?

01 February 2021 3,264 2 View

Stimulation system for field recordings?

We have a stimulation isolator (A365). When we put the stimulus and recording electrodes into solution (even with miliq water), some current came out (see attached). The current was gone when the...

26 August 2020 9,139 2 View

What is the proper aldosterone and CORT supplement in ADX mice?

Hi, I am struggling with the stress experiements in ADX mice. i tried 15ug/ml cort in the drinking water for ADX mice, however, the basal plasma cort are much higher than sham controls( 260ng/ml...

21 June 2020 386 1 View

What's the reason for the crossover of illuminated and dark JV curve in CIGS solar cells?

The distinct crossover anomalies are observed in my recent samples. It's explained in the book that there might be a barrier at the interface or in the absorber's near interface region.The barrier...

29 May 2020 675 4 View

How should I hydroxylate tungsten powder for organosilane grafting?

I am having trouble depositing MPTMS/APTES onto tungsten and I am concerned there are not enough hydroxyl groups on the powder surface. So far I have been exposing tungsten to water to hydroxylate...

04 February 2020 4,677 2 View

How to calculate effect size in R with Metafor package?

07 October 2019 8,027 3 View

Could I extract RANK/RANKL/OPG mRNA from peripheral blood samples?

I want to know that whether it is possible to extract RANK/RANKL/OPG mRNA from peripheral blood samples instead of tissue homogenates. Thanks a lot.

07 September 2019 7,431 3 View

How to calculate the area of coverage hole of a disc tessellation?

The locations (centre coordinate x,y) and radii of all the circles are given. How to get the area of the coverage hole (area in green in the figure) ? Radii may not equal to each other. It is a...

11 June 2019 5,127 15 View

For WB, if there were DNA in my protein sample, how can I do?

Hi, everyone, I am working on transcriptional factor, I want to define what protein bind to my specific DNA, after I got the sample that bound to DNA, and use SDS buffer to boil it, I can find the...

24 June 2018 4,582 5 View

How the overall score is related to their subscale scores?

This is the third section of Items Decomposition: How to structure the Scores and Items. This section describes, using the graphical representation, how the score associated with all items in a...

07 June 2018 8,942 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View