I am growing a suspension cell culture and wondered if the type of flask matters for these types of cells. I.e. Should I only use suspension culture flasks or can I also use tissue culture-treated flasks?
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Hello everyone! I am currently exploring the performance of large models in understanding knowledge in specific domains, and attempting to construct a knowledge framework similar to what...
05 August 2024 5,729 2 View
Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...
03 August 2024 5,358 0 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
N=6 Comparing pre and post test likert scale responses. Participants are mix of practicing & preservice teachers.
30 July 2024 7,233 4 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...
16 June 2024 3,752 0 View
Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...
09 June 2024 6,311 0 View
I was wondering if you can move immunofluorescence ready sample that is in the form of suspension on 96-well plate to another well plate. Is that possible?
14 May 2024 596 1 View
Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...
05 May 2024 3,316 1 View
i am doing THP-1 suspension culture and using cefotaxim as a antibiotic but after 4 passag my cell count was decline, so is it becuase cefotaxim my cell is decline
01 May 2024 9,779 3 View
I am struggling with manual counting using neubauer chamber. We have automated counter as well. Manual counting shows lesser cell density while automated ones show more. Mostly people say manual...
30 April 2024 278 0 View
In the BCA assay, are there any differences between the reported protein concentration directly from the cell suspension and from the protein extracted solution of the cells by RIPA buffer?
30 March 2024 8,359 0 View
Hi everyone, I prepared nanoparticles from two different green sources. After centrifugation, washing, and drying I got a fine powder of pure nanoparticles. I want to determine the MIC value of...
21 March 2024 8,097 1 View