Hi everyone, I prepared nanoparticles from two different green sources. After centrifugation, washing, and drying I got a fine powder of pure nanoparticles. I want to determine the MIC value of synthesized nanoparticles against bacteria but I don't know how to make good suspension of nanopowder in deionized water or DMSO without sonication. Please suggest any other method or can I use a solution of freshly prepared nanoparticles without centrifugation or drying for MIC determination?