Mubasher Iqbal

4 Questions 3 Answers 0 Followers

Questions related from Mubasher Iqbal

What is the better practice for adding IL2 to cell culture media?

I am working on a project in which we obtain lymphocytes through Ficoll density gradient separation and then culture them for ~3 weeks. Naturally, we add IL2 to our cell culture media. There are...

29 February 2024 1,248 1 View

Does the flask type matter for suspension cell culture?

I am growing a suspension cell culture and wondered if the type of flask matters for these types of cells. I.e. Should I only use suspension culture flasks or can I also use tissue culture-treated...

26 September 2023 5,665 0 View

Spiking cancer cells in whole blood?

I am conducting an experiment where I am putting cultured cancer cells (B-ALL line) in whole blood and running them through a microfluidic device. I stain the cells with DAPI and DiI (cell...

19 September 2023 7,680 1 View

What is the protocol for detection of mycoplasma in suspension cultures?

I am growing some leukemia cell lines (MV411, CEM) and suspect that I might have mycoplasma contamination. I see black dots in my cell culture, and I am unsure if it is cell debris or mycoplasma....

17 August 2023 3,184 5 View