Does tetracycline free and charcoal stripped FBS exist?

15 August 2023 1 781 Report

I need to conduct an experiment by dosing cells with steroid hormones, and the cell line is a tet-inducible overexpression model. Please let me know if you have experience with this and if it is commercially available or possible to make.

Sabine Strehl

There is either Tet-Free or Charcoal-Stripped FBS available.

https://www.thermofisher.com/at/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum.html

Tet-System Approved FBS (Tet-Free FBS)

https://www.thermofisher.com/at/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum/tet-system-approved-fbs.html

Charcoal-Stripped FBS

https://www.thermofisher.com/at/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum/charcoal-stripped-fbs.html

Not sure, but Dialyzed FBS might be an alternative

https://www.thermofisher.com/at/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum/dialyzed-fbs.html

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