If I am measuring the fluorescence of a substrate at 350nm ex but another compound in the reaction has an 287nm ex will that interfere?

I am running an enzyme assay that measures the fluorescence of a cleaved substrate at 360nm ex and 445em. I also have a compound in the reaction that can be fluorescent if excited at 287nm. Will...

08 October 2020 4,832 5 View

Has anyone been able to quantify iodide using an AS28 column on an Integrion IC with hydroxide eluent?

20 August 2020 2,600 1 View

Correct way to mesure the BER in a simulation of a time variant frecuency selective fading?

I am traying to simulate a frequency selective fading channel in Matlab and I have faced with some issue/questions during the implementation. It is a DSSS (BPSK) modulation and the code is...

26 January 2020 3,335 4 View

EFA Factors determined by Eigenvalue Not allowing matrix to load?

I am using an instrument that has been found to be reliable and valid twice in published literature. However, the focused population is different (i.e. different countries, similar careers). The...

01 December 2019 753 3 View

What is the difference between quercetin and quercetin dihydrate?

We purchased quercetin powder from China and we don't know if we should use the molecular weight of just quercetin or quercetin dihydrate since it is already in powder form. Is there a difference?

12 March 2016 7,923 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View