I have stored my LC540 cells in liquid nitrogen 3 months back and wen I try to retreive cells die. Can anyone help me out. S soon as I take vial out from LN2 I place them in 37 deg C waterbath and add complete medium to it and seed them. After 4 hrs I see cells attached from the next day my cells start dying.
Hi Arun,
Personally, I don't have experience working with lc540 cells but our team has developed a innovative solution for room temperature storage of different cell types, i.e. MSCs, PBMCs, hepatocytes. Do you cryopreserve your cells for short-term storage?
I m not experienced with the LC540 but, Its fine to store up-to 3 months cells in Liquied nitrogen [1]. The cells are dying maybe due to the process , when you try to retreive I think.
Article Long-term storage in liquid nitrogen does not affect cell vi...
(1)
Dear Arun,
I have one similer experience in cell culture when using expired medium suppliment. If your cell in good cryopreservation manner it will normally start grow after releasing. But, after 4 hrs may not enough to see this conditions. I suggest you to check your medium conditions whether it can give sufficient nutrients for cell growing.
Ex. Expired date, supplements, storage conditions.
Good luck
- Badges
- Science method