Arun Kannan

10 Questions 13 Answers 0 Followers

Questions related from Arun Kannan

How to Induce inflammation invitro in H460 cells?

I need to induce inflammation model invitro in H460 cells. I did I duce cells with 20ugper ml and 40ug per ml for 24 and 48hrs do not see any increase in cytokines il6 il8 il1b and TNFa. Please,...

03 February 2023 562 0 View

Does stored lc540 cells have negative effect when stored in liquid nitrogen?

I have stored my LC540 cells in liquid nitrogen 3 months back and wen I try to retreive cells die. Can anyone help me out. S soon as I take vial out from LN2 I place them in 37 deg C waterbath and...

06 June 2020 758 3 View

Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

02 March 2020 2,133 4 View

Oocyte swelling assay detail protocol. Aquaporin inhibition studies. Any suggestions for other studies?

I am completely unaware of swelling assay as i am from a different back ground. I have a compound which i hypothesize that it will inhibit aquaporin. I am not sure that it would make any gene or...

09 March 2018 2,524 0 View

Does ip injection with STZ in wistar rats does increase body weight?

I did inject STZ at 60 mg/kg bw and it's glucose levels has increased up to 533 mg/dl. When i initially started before injection of STZ its weight was around 155 g and its 2 weeks almost the...

06 September 2017 407 9 View

Doubt regarding diabetic infertility? Is diabetes the ause of infertility

A diabetic patient either type I or type II is on regular medication and has kept his glucose levels in normal condition will he become infertile. And if he becomes infertile can we say that it...

26 October 2016 7,885 3 View

I am planning to start up with 2D proteomic analysis on rat testis. If do anyone have reference 2D gel on the same any one could help me?

The pH range of my gel would be 3 to 10.

11 August 2015 9,434 4 View

How reliable is an analysis of pancreas on stretozotocin induced diabetic rats?

We know that wen rat is given with streptozotocin beta cells are damaged , further research on pancreas shows it holds good?

28 February 2014 7,954 1 View

How do you mimic diabetes conditions in LC-540 (lyedig cell)?

I have seen many papers in past using LC-540 grown in high low glucose, but I haven't seen any papers in recent years doing so. I just need to clarify that if i can use high glucose and low...

27 February 2014 9,157 0 View

Does PCR once standardized using rat liver differ for another animal? And if yes why is that so?

I have just received rat specific primers, and I have optimized it to my lab conditions and sequenced it fine. My question now I have six rats in each group so will the PCR results in the sense...

11 February 2014 8,167 2 View