Arun Kannan

6 Questions 9 Answers 0 Followers

Questions related from Arun Kannan

How to Induce inflammation invitro in H460 cells?

I need to induce inflammation model invitro in H460 cells. I did I duce cells with 20ugper ml and 40ug per ml for 24 and 48hrs do not see any increase in cytokines il6 il8 il1b and TNFa. Please,...

03 February 2023 523 0 View

Does stored lc540 cells have negative effect when stored in liquid nitrogen?

I have stored my LC540 cells in liquid nitrogen 3 months back and wen I try to retreive cells die. Can anyone help me out. S soon as I take vial out from LN2 I place them in 37 deg C waterbath and...

06 June 2020 703 3 View

Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

02 March 2020 2,091 4 View

Doubt regarding diabetic infertility? Is diabetes the ause of infertility

A diabetic patient either type I or type II is on regular medication and has kept his glucose levels in normal condition will he become infertile. And if he becomes infertile can we say that it...

26 October 2016 7,842 3 View

How reliable is an analysis of pancreas on stretozotocin induced diabetic rats?

We know that wen rat is given with streptozotocin beta cells are damaged , further research on pancreas shows it holds good?

28 February 2014 7,910 1 View

Does PCR once standardized using rat liver differ for another animal? And if yes why is that so?

I have just received rat specific primers, and I have optimized it to my lab conditions and sequenced it fine. My question now I have six rats in each group so will the PCR results in the sense...

11 February 2014 8,125 2 View