Hello, I wanted to do a reverse transcript for the subsequent realization of a sybr green qPCR. Classically I prepared my samples by mixing them with water, dNTPs, Random primers and the RNA of interest and finally at the time of denaturing them I realized that the thermocycler was out of order. My question is: can the samples be kept at -20 ° C until the thermocycler is operational, or do I have to start again for better RT? Thank you
It is recommended to keep the prepared samples at 4oC, for a short period of time. Some companies already recommend to store the 2*MMX at 4oC since the mixture will be stable.
About prolonged storage more than a couple of days, never had the chance to test it and I can not recommend anything.
Hi Fatou,
it's nor recommended but you can perfectly store the reaction mixture at -20°C for some times. you just need to be sure to respect time and temperature just to be sure the enzymes are not activated and in good shape. I've already done that for sequencing, it was fine.
fred
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