Hello.
I would like to ask some questions regarding technique of oocyte aspiration from live donor mares.
The mares are sedated, without epidural anaesthesia. We are using 18G needle connected to syringe. We tried to flush the follicle several times (5-8). First, we used gentle pressure. Second we used rough pressure. During flushing I was sometimes able to move with the needle/ovary to scratch the follicle which should better release the oocyte. Of course this was not the case in all mares. Our successful rate is for the present really low. I had the chance to work with the ovary from slaughter house, because I wanted to see what is happening with the follicle when I work with live donors and I regarding what I saw the pressure of fluid which we pushed back to the follicle should be really gentle.
My questions are:
1/Do you think we should focus on “scratching” the follicle every time (all size categories)?
2/Do you think follicles can leak out from the follicle through the hole around the needle?
3/Have somebody tried to inject hCG directly to the follicle and harvest the follicles some hours later?
4/Is it better to rotate the ovary so I can reach all the >2 follicles or is it better to inject the needle through the ovary?
Thank you very much for your answers.
Best regards,
Jiri
Hi Jiri.
You could contact one of the following, a group in Germany that have good experience with oocyte collection from mares:
Prof. Dr. Wilhelm Kanitz ([email protected])
Dr. Helmut. Torner ( [email protected] )
Dr. Frank Schneider ([email protected])
Wilhelm-Stahl-Allee 2
18196 Dummerstorf
Tel. 038208 68751
Fax 038208 68752
Homepage: http://www.fbn-dummerstorf.de
Good luck
Please contact Dr. Catalina Velez ([email protected]) from Medellín, Colombia.
Hi Juan.
Thank you very much.
I was doing OPU just yesterday and we are perfect until "scratching" the follicular wall when the needle is injected. I think, that is the main reason we are getting so low numbers of oocytes.
Please, does somebody have experience with this "scratching"?
Thank you,
Jiri
Hi Jiri,
I do have broas experience in mare OPU, in case you have not contacted Catalina Velez and need any further advice, just let me know. Regarding the follicle size, I can tell you that follicles around 10-20 mm are the easiest, and you have to try to aspirate everything you can possibly see. In general, low recovery rates are quite normal at the beginning, but the more experienc the person on the needle and the one on the ovary get, the better the recovery rate. Kind regards, and don't hesitate to contact me in case you need anything. Regards,
Bea
Dear Beatriz.
Thank you very much for your answer and recommendation.
We are quite ok with the recovery rate now (approximately 50%) so I think it won't increase that much from this point. On the other hand we would like to start with maturation and ICSI and this will be quite hard. Well we do work with pig and bovine oocytes at our lab but...we need to think out some maturation scheme and this will be quite challenging. Do you also maturate and perfrom ICSI?
Regards,
Jiri
Hi,
yes, we are currently doing equine oocyte maturation and I can help you with that. Unfortunately ICSI is not still available to us yet, but I have some theoretical knowledge that can hel you at the beginning. Don't hesitate to contact me at [email protected] in case you need it. Good luck!
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