What is the difference between research gap and problem statement in academic studies?

Often postgraduate students are confused about the difference between the research gap and problem statement. Please discuss.

05 August 2020 4,235 2 View

How energy transfer from C-dots influence of band gap of p-type semiconductor?

how energy transfer from carbon dots to p-type semiconductor decreases band gap?

20 June 2019 5,946 1 View

What is the greatest challenge to population controlefforts?

The global population is exploding at an alarming rate, particularly in Sub-Sahara Region of Africa and South-east Asia regions. The different factors (to be proved or refuted by research) are the...

21 April 2019 5,062 3 View

What is the NiO@C-dots XRD ,TEM and FTIR image look like?

what is the NiO@C-dots XRD ,TEM and FTIR image look like? I need some explanation.

07 March 2018 6,668 3 View

Could anyone help me how to calculate the energy dissipated during 30 g of ice crystal formation in a closed refrigerator of inside T, -20 0C?

29 July 2016 8,295 3 View

Is it possible to exteract two exposure OR for one outcome from one study for meta analysis?

01 January 1970 8,239 1 View

Is it possible to quantify and model the role of pollination on ecosystem health and agricultural productivity?

Incomplete pollination on ecosystem productivity, Excessive use of pesticides and pollinators, etc.

01 January 1970 4,583 7 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View