Sintayehu Fekadu

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Questions related from Sintayehu Fekadu

I am trying ligase based cloning for GOI using T4 DNA ligase. Transformation was succeeded with insignificant background, but no insert, why?

Recently trying ligase based cloning using T4 ligation. I digested the donor plasmid and linearize the recipient with the same enzyme (double digestion) to generate cohesive ends. The insert was...

30 December 2019 4,781 4 View

Does PEI transfection effeciency affected by long storage at -80oC?

Recently I am doing experiment for EBV supernatnat (virus) collection from AGS+ cells using PEI solution. For the experiment I used PEI stored at -80oC.After collection I checked the virus titer...

12 September 2017 3,915 2 View