Anyone can suggest a chemical coating for silicon substrate to make a water contact angle between 30-40?

I prefer a silane with low boiling point as I am going to deposit it in the vapor phase (chemical vapor deposition). I have tried TMS (Chlorotrimethylsilane) using CVD on Si wafer and I got water...

13 August 2018 2,378 3 View

Does anyone know what is the best procedure for coating a silicon wafer with APTES (3-Aminopropyl)triethoxysilane) in vapor phase?

Thanks in advance

13 August 2018 9,792 4 View

Whats the reason limiting the fraction of filled pores (upon immersing in liquid) when increasing the thickness of a porous film (e.g. IOF)?

18 April 2017 4,675 1 View

What should be done when the two tests used in fitting probability distributions give two highly different p-values?

Dear Researchers, I am using ARENA Input Analyzer to get the best probability distribution from data (attached). The resulted p-value of Chi Square Test < 0.005 and for K-S Test > 0.15....

05 October 2016 9,094 3 View

Should the weights be integrated (=100%) in the weighted sum objective function?

01 January 1970 8,216 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

The lipid bilayer broken down and form micelle within 1ns MD simulation. How to simulate lipid bilayer without breaking bilayer?

To simulate the lipid bilayer (10-10, 25-25 so on) from CHARMM-GUI membrane builder, we used to simulate it at various force field (AMBER14, AMBER03, AMBER14IPQ, AMBER15FB, NOVA, YAMBER) at water...

26 January 2021 7,816 2 View

SEM/TEM/AFM which analysis should be performed for spray dried solid lipid nanoparticles?

Can anybody please suggest which analytical technique would give me the best result for the size and shape of solid lipid nanoparticles SEM or TEM or AFM. The nanoparticles are synthesized by...

21 January 2021 7,991 6 View

Why the lipid accumulation levels in my 3T3L1 does not match with my adipogenic and lipogenic gene expression levels?

I am using the 3T3-L1 cell line to discover the adipogenic effects of a natural compound and its derivatives. I conducted a lipid accumulation assay using fluorescent Nile Red dye (Adipored) for...

10 January 2021 4,360 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

Does someone have tips on how to reduce the amount of entrapped oil in the membrane of GUVs with the Phase Transfer Method?

I'm trying to prepare GUVs using the phase-transfer method but apparently I'm getting residual oil entrapped in the bilayer. Did anyone manage to overcome this? Basicaly, I'm following the...

24 November 2020 2,962 1 View

How can I correct my electrostatic potential figure to be symmetric?

hi. I have done electrostatic potential analysis on a symmetric bilayer contains 10% Cholesterol but I've got this figure and I thought there is a problem with that as the potential is not...

09 November 2020 7,137 1 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

What is needed for Olive Oil Lipid Extraction?

I am doing my thesis and would like to extract triglycerides from Olives or Olive Oil. I will be analysing the amount of triglycerides that are present in the olives or Olive Oil I will be...

01 November 2020 8,314 5 View