I tried to find publications about anticancer effect of MTB cellular and extracellular protein i found only one paper concerned with this topic. Also i would like to know what is the recommended tests to study this point ?
Congratulations for the great work in the field of antimycobacterial agents!
I am working on MTBDRsl to test second line-drug resistance in MTB resistant to first-line drug , but i am wondering if MTB show susceptible result to first-line drugs and resistant to second-line...
06 July 2016 885 2 View
I f i used Microarray to study transcripteome of bacteria under two different conditions i need to confirm with RT-PCR. Does Next generation sequencing also need confirmation?
03 April 2016 9,279 2 View
I am trying to synthesize surface modified biogenic silver nanoparticles and would like to know how their surface changed how this modification affect on their biological activity and i am...
03 April 2016 5,117 1 View
Often i have confusion in describing study and can not differentiate between types of studies for example cross-sectional , retrospective study , longitudinal and descriptive study ..........
31 December 2015 5,991 2 View
How can I interpret rpoB gene sequencing results? I have sequenced rpoB gene of 10 MDR Mycobacterium tuberculosis isolates, could anyone help me to interpret these results using bioinformatics...
09 October 2014 2,930 6 View
i am beginner in bioinformatics field i am looking for studying online master degree in microbiology bioinformatics , could anyone help me to find university provide this online service?
09 October 2014 2,345 1 View
I have two Mycobacterium cultures which are grown under two different stress conditions and I want to study transcripteome profile of each one.
08 September 2014 3,027 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View