Can Mycobacterium tuberculosis isolate be sensitive to first-line drug and resistant to second-line drugs in the same time??

06 July 2016 796 2 View

Does NGS need confirmation by another method for transcripteom study?

I f i used Microarray to study transcripteome of bacteria under two different conditions i need to confirm with RT-PCR. Does Next generation sequencing also need confirmation?

03 April 2016 9,184 2 View

Can anyone suggest tests to study biological activity and surface changes of surface modified Silver nanoparticles?

03 April 2016 5,026 1 View

What is the difference between types of studies in diagnostic test evaluation and epidemiological studies?

31 December 2015 5,893 2 View

Could anyone help me to interpret rpoB gene sequencing results?

How can I interpret rpoB gene sequencing results? I have sequenced rpoB gene of 10 MDR Mycobacterium tuberculosis isolates, could anyone help me to interpret these results using bioinformatics...

09 October 2014 2,829 6 View

Could anyone help me to find university provide certified bioinformatics master online?

09 October 2014 2,251 1 View

Could anyone recommend a company or lab, providing service of Mycobacterium tuberculosis gene expression by micro-array and how much the sample cost?

I have two Mycobacterium cultures which are grown under two different stress conditions and I want to study transcripteome profile of each one.

08 September 2014 2,933 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View