Does NGS need confirmation by another method for transcripteom study?

I f i used Microarray to study transcripteome of bacteria under two different conditions i need to confirm with RT-PCR. Does Next generation sequencing also need confirmation?

03 April 2016 9,184 2 View

Can anyone suggest tests to study biological activity and surface changes of surface modified Silver nanoparticles?

03 April 2016 5,026 1 View

Does Mycobacterium tuberculosis extra-cellular or cellular protein has anticancer effect?

I tried to find publications about anticancer effect of MTB cellular and extracellular protein i found only one paper concerned with this topic. Also i would like to know what is the recommended...

02 March 2016 7,580 1 View

What is the difference between types of studies in diagnostic test evaluation and epidemiological studies?

31 December 2015 5,893 2 View

Could anyone help me to interpret rpoB gene sequencing results?

How can I interpret rpoB gene sequencing results? I have sequenced rpoB gene of 10 MDR Mycobacterium tuberculosis isolates, could anyone help me to interpret these results using bioinformatics...

09 October 2014 2,829 6 View

Could anyone help me to find university provide certified bioinformatics master online?

09 October 2014 2,251 1 View

Could anyone recommend a company or lab, providing service of Mycobacterium tuberculosis gene expression by micro-array and how much the sample cost?

I have two Mycobacterium cultures which are grown under two different stress conditions and I want to study transcripteome profile of each one.

08 September 2014 2,933 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View