Can Mycobacterium tuberculosis isolate be sensitive to first-line drug and resistant to second-line drugs in the same time??

06 July 2016 796 2 View

Can anyone suggest tests to study biological activity and surface changes of surface modified Silver nanoparticles?

03 April 2016 5,026 1 View

Does Mycobacterium tuberculosis extra-cellular or cellular protein has anticancer effect?

I tried to find publications about anticancer effect of MTB cellular and extracellular protein i found only one paper concerned with this topic. Also i would like to know what is the recommended...

02 March 2016 7,580 1 View

What is the difference between types of studies in diagnostic test evaluation and epidemiological studies?

31 December 2015 5,893 2 View

Could anyone help me to interpret rpoB gene sequencing results?

How can I interpret rpoB gene sequencing results? I have sequenced rpoB gene of 10 MDR Mycobacterium tuberculosis isolates, could anyone help me to interpret these results using bioinformatics...

09 October 2014 2,829 6 View

Could anyone help me to find university provide certified bioinformatics master online?

09 October 2014 2,251 1 View

Could anyone recommend a company or lab, providing service of Mycobacterium tuberculosis gene expression by micro-array and how much the sample cost?

I have two Mycobacterium cultures which are grown under two different stress conditions and I want to study transcripteome profile of each one.

08 September 2014 2,933 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View