We have BXPC-3 human pancreatic cell line which had mycoplsma contamination, we treated them with plasmocin for 2 weeks and now the morphology of the cells totally changed. They used to be polygonal and formed tight colonies at lower seeding densities, not they are spindle shaped and do not form colonies at all. We sent the DNA sample for authentication and it matched so there is no cross contamination with other cell lines and the mycoplasma result came negative, but the cells don't look like BXPC-3 anymore.
Please help us trouble shoot this issue.
Thank you very much!
For our cell cultures, plasmocin has not resulted in any morphology change. Our cells look and behave the same as before. You might have lost the original population of cells and the current cell culture is the growth of less represented cells of original cell culture. Also what kind of DNA authentication have you done, is it species based or tumor cell specific based. You can check pancreatic cells specific rna expression, which is functionally more accurate than DNA (DNA is same in different tissues but functionality is different). I hope it helps!
Hi Payal,
Our core authenticates only human cell lines, and it is also tumor specific.
Is there any way to reverse this change?
Thank you very much.
Sincerely,
Lakshmi Shree
I don't know if you can. However I was looking at ATCC site, your cell lines supposedly looks different at different density. https://www.atcc.org/Products/All/CRL-1687.aspx#characteristics Check this link, if it helps!
I have seen in my experiment that the shape and cytoplasmic projection of human cells had changed when the sterilized broth media of 24 h cultured pathogenic and non pathogenic bacteria were added.
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