Lakshmi Shree K Mahadevan

4 Questions 6 Answers 0 Followers

Questions related from Lakshmi Shree K Mahadevan

How different is western blot protocol for phosphorylated protein from regular western blot?

Hello everyone, I am trying to probe for phosphorylated ERKthrough western blot. How different is the protocol from regular western blot? I looked up on abcam and they ask to use bsa instead of...

31 August 2017 2,820 9 View

Can anyone give me a protocol for stable luciferase transfection of murine cell lines?

I want to do a stable luciferase transfection of murine PDAC (PANC02) and colorectal (CT26) cell lines. Please help me find where to obtain the protocol and reagents. Thank you. Sincerely, Lakshmi.

06 April 2017 2,637 4 View

Does morphology of tumor cells change after mycoplasma treatment?

We have BXPC-3 human pancreatic cell line which had mycoplsma contamination, we treated them with plasmocin for 2 weeks and now the morphology of the cells totally changed. They used to be...

18 February 2017 9,589 4 View

Whats is the maximum concentration of NaOH that is not toxic to cell cultures?

I am using 1M NaOH solution to dissolve a drug. I am trying to estimate its IC50 using MTT assay. The data sheet says that the drug is soluble in 1M Naoh at 50mg/ml. Has anyone had experience...

19 September 2016 1,220 6 View