Dear Maryam,

Heparin about 0.1 to 0.0016 Units per reaction mixture (50 ul) suppresses DNA amplification in a concentration dependent fashion hence care should be taken when heparinized blood is used for PCR.  However, when the patient takes heparin as a medication and you take the blood from heparinized patient the same can happen. 

Omer

@ omar Iqbal, Thanks for your helpful guide. 

Maryam,

Thank you for the thank you. 

Omer