Does eosin stains collagen in IHCs in typical H&E staining?

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Erik Kouba

H&E stains (eosin) collagen of different composition that shows characteristic patterns when examined under light polarization or fluorescent microscope. For example, the collagen of DFSP vs. dermatofibroma shows opposite polarization. I don’t use fluorescent microscopy but I attached an article you may find interesting. Also try this DOI for studies of dermal collagen and polarized light.

doi: 10.1097/DAD.0000000000000550

A particular useful website with links to relevant histology and staining protocols, etc

http://www.ihcworld.com/index.htm