What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Hello friends, happy evening !! I am just wondering if you would know where I can find the (ALTS) altruism scale by S.N.Rai and S Singh to download ?

ALTS is an altruism scale developed by S.N Rai and S.Singh.

02 February 2021 7,243 2 View

What does a negative PSNR value between am image and its filtered version indicate?

I understand that because of the log in the equation of PSNR, a negative value is obtained if the value is less than 1. But what does the negative value of PSNR imply?

09 November 2020 392 3 View

What is neutrosophic similarity score (NSS) used for segmentation in image processing? How can it be implemented in Matlab?

Neutrosophic Similarity score is similar to Fuzzy logic. Few papers report using it for image segmentation but implementation details are not provided. If any material regarding this on how it can...

05 November 2020 2,859 3 View

What do you think about Herd Immunity ? Is it Attainable or Unattainable?

Researchers think Vaccine is the only solution .

21 September 2020 8,155 5 View

Which option (SFA-Cross Or DEA-DMU) is best to measure the individual efficiency (for a sample size of 450 farmers)?

I am working on a set of data, collected from a field survey. I am looking forward to measure the the level of technical efficiency of type of irrigation channels (Solar pump set/ Diesel pump set...

06 August 2020 2,510 3 View

How to design a circuit for Reconfigurable antenna using PIN diode in hardware?

I have simulated the reconfigurable antenna using PIN diodes using HFSS software.Now for the hardware implementation i am not getting how to give current to the diode. Should i use RLC circuit to...

18 December 2019 6,702 3 View

How can I combine four different items/questions to one variable?

I have four questions and I want to make it one variable. The one variable I wanted to make is work-family conflict. The coding I have 1= several times a week, 2= several times a month, 3= once or...

28 January 2017 9,980 8 View

Standard method for total RNA isolation of bacteria

total RNA isolation from antibiotic resistant and susceptible bacteria

02 October 2016 4,006 2 View

How to reconstitute SiRNA?

I want to reconstitute my SiRNA. Can i reconstitute it in DEPC treated water or 1X SiRNA buffer is compulsory. If yes, I also want to know the exact composition of this buffer. Can anyone help me...

19 September 2016 7,965 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View