Dear colleagues,
May I seek your kind guidance regarding how to do colocalization of actin-binding proteins at stress fibers specifically? I do not want to do coloc of the whole field or of whole-cell but rather I need to look for colocalization of my target proteins on actin stress fibers only. I would appreciate it if you would suggest to me some software regarding this.
Thanks in advance
Hi, the colocalisation part is straightforward, the critical bit is to segment the stress fibres. Once you have a segmented image of stress fibres you can just use any colocalisation plugin in FIJI, e.g. JACoP. If you attach a typical image, I or somebody else may be able to suggest an approach how to segment the stress fibres
Dear Radek Machan , thank you for your response. Can you please suggest me how to segment stress fibers? Please find one of my images attached with this response. I guess, I can manage with JACop afterward.
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