What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Hello friends, happy evening !! I am just wondering if you would know where I can find the (ALTS) altruism scale by S.N.Rai and S Singh to download ?

ALTS is an altruism scale developed by S.N Rai and S.Singh.

02 February 2021 7,243 2 View

What does a negative PSNR value between am image and its filtered version indicate?

I understand that because of the log in the equation of PSNR, a negative value is obtained if the value is less than 1. But what does the negative value of PSNR imply?

09 November 2020 392 3 View

What is neutrosophic similarity score (NSS) used for segmentation in image processing? How can it be implemented in Matlab?

Neutrosophic Similarity score is similar to Fuzzy logic. Few papers report using it for image segmentation but implementation details are not provided. If any material regarding this on how it can...

05 November 2020 2,859 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

What do you think about Herd Immunity ? Is it Attainable or Unattainable?

Researchers think Vaccine is the only solution .

21 September 2020 8,155 5 View

Is there a method to quantify a particular protein of interest from the crude extract?

I want to quantify a protein of ~ 7 kDa ( not a recombinant or amplified) from the crude protein extract. The sequence of the protein is known and has only one UV absorbing aminoacid.

04 February 2020 7,574 4 View

Usage of steel slag in GPC offers strength ?

Steel slag is used as partial replacement of fine aggregate and coarse aggregate. Does it increases strength of Geopolymer concrete (GPC)? Is there any relevant articles available ?

02 February 2020 3,245 7 View

How to design a circuit for Reconfigurable antenna using PIN diode in hardware?

I have simulated the reconfigurable antenna using PIN diodes using HFSS software.Now for the hardware implementation i am not getting how to give current to the diode. Should i use RLC circuit to...

18 December 2019 6,702 3 View

How to set the axis of fft2 in matlab for spatial wave?

I have some data of radiances over latitude and longitude. When we do fft, the frequency window is from -fs/2 to fs/2. But, when we do fft2, what will be in the x axis and y axis? -spatial...

16 November 2019 8,956 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View