Sushma Dagar

4 Questions 31 Answers 0 Followers

Questions related from Sushma Dagar

What percentage of Agarose gel should I run to see the difference of 40bp?

Hi Everyone, I have the pcr product size of 250 and 290 base pairs. I have tried running 1.5% and 2% gel at 90V for 1 hour. They seem to appear at the same site. How can I resolve them better to...

14 October 2015 7,441 3 View

Does anyone have experience with analysis of Structured illumination microscopic images?

Hi, I am trying to find a suitable thresholding method for the SIM images. I have done ICC on mouse cortical neurons and then have taken few image using Zeiss Elyra PS-1 microscope. I am doing...

05 October 2015 8,202 15 View

Can anyone help me with pure primary neuronal culture?

I am doing pure cortical neuron culture from mice E18. I use Poly-L-Ornithine for coating of cover slips and grow neurons for 2 weeks. Initially, everything was working fine. But now facing...

29 July 2014 9,696 39 View

Does degeneration of photoreceptors cause change in the structure of a protein?

I am doing western blotting for synaptic vesicle protein and it gives double band with smeared pattern (manufacturers details). I am also getting the same pattern in wild type mouse but this...

29 May 2013 1,552 3 View