Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

How does climate change affect birds (shorebirds)?

I m recently investigating the impact of climate change on shorebird population trends, I would like to know what is the impact of fluctuations in variables such as sea surface temperature, global...

10 November 2020 9,293 15 View

Can I use the EPIC-Oxford FFQ for free?

Designing my PhD project and need to use an FFQ, but we don't have any funding for this part of the project.

09 November 2020 4,541 1 View

Is there a survey tool (similar to REDCap) through which we can send an anonymous survey and restrict respondents to one survey each?

I am working on a research survey where I need to send it out to a group of users and track in (anonymous) responses. I need to restrict users to completing the survey only once. I do not think...

06 August 2020 918 2 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

How many users can i put in the same vsat broadband plan?

sorry if i wasn't able to explain very well for example, i am in a country that doesn't have satellite internet service, and would like to be the first provider for this service. lets say i have a...

05 July 2020 4,637 1 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

Inrush control when Transformers are Energized at Solar Renewable is best achieved how?

Increasing use of solar renewable resources or farms on weak remote locations are causing significant power quality and voltage notch issues with connecting utilities. High penetration of these...

17 August 2019 7,097 2 View

Glass microballoons from sodium silicate solution?

I once produced microballoons from sodium silicate solution (water glass). does anyone have experience in manufacturing these, are articles you could point me in the direction of? thanks

01 July 2019 8,764 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

No title

28 February 2021 9,936 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View