Why the contaminated colony grow around the plate?

Doing the antibacterial test, the unknown colony grows around the plate. E. coli and antibacterial regent were mixed with LB, PBS and EDTA. Not likely the contamination was from the LB, PBS or...

18 May 2020 1,311 3 View

In geometric morphometrics, is setting a scale necessary when you place landmark points on objects?

I am confused about setting a scale when you place landmarks on objects because I read that when you do landmark placement on tps you can set the scale. I thought setting scale was unnecessary...

08 April 2019 8,750 6 View

Why is my Western Blot not producing the desired result?

Hey guys, I am having an issue where I am not getting the correct results on my Westerns that I am running. When I run my Western for VGLUT1 from mouse hippocampi samples, with an antibody...

12 October 2018 7,914 3 View

Problems staining astrocytes (C8-S) cell line with GFAP?

29 June 2018 8,233 3 View

Anyone aware of literature studying the impact of past collaborative experiences on a firm/organization's current collaboration choices or attitudes?

Dear all, For a research project I'm currently working on I am looking for literature that documents how a firm/organization's past experiences (positive or negative) in collaboration influences...

17 June 2018 5,284 3 View

Freezing conditioned media from activated microglial cell cultures?

Hello Everyone, I am doing an experiment where I induce C8-B4 microglial cells with 10 micrograms of LPS at different volumes. The goal is for these cells to be activated and release glutamate....

17 May 2018 5,234 3 View

What are ways to store collected soil samples for microbiological research? What are good containers of such soil samples?

I've seen ways to collect soil samples, but I would like to know if there are standard procedures for collecting soil samples for microbiological study.

07 March 2018 6,686 6 View

Any experiences with using persona's in a design process with the goal of allowing end-users to talk about sensitive issues through a 3rd person view?

21 March 2017 6,601 3 View

How much can the band gap value of nanoparticles differ from the bulk?

I am working on CdS nanoparticles. I got a band gap value of 5.89 eV whereas the bandgap value of my bulk is 2.42 eV. I am using double distilled water as my solvent in UV studies. Why did I get...

10 April 2014 756 6 View

What is the colour of CdSe nanoparticles?

I am getting a white colour. Is this correct?

08 April 2014 4,218 10 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View