Jasper F. Nies

2 Questions 3 Answers 0 Followers

Questions related from Jasper F. Nies

Flow Cytometry Intracellular Cytokine Staining - What is this "Double-Positive-Smear"?

Hi everyone, I am staining leukocytes isolated from mouse kidneys in flow cytometry. I stimulated the cells with PMA/Ionomycin + BFA for 4 hrs in T-cell medium (RPMI + Pen/Strep + 10 % FCS + 50...

23 April 2024 5,754 3 View

Does centrifuging EDTA blood with 3500 g alter protein levels in plasma?

I accidentially centrifuged my EDTA blood samples with 3500 g instead of 3500 rpm (roughly 1700 g) for 10 minutes. We want to measure the level of cytokines in the plasma. Do you think that the...

23 February 2017 7,899 4 View