Dear friend,
I am wondering any friend has tried/is trying to use BioID methodology to screen for proximate proteins by use yeast. If so, or if you know something about yeast BioID, please may you share the knowledge with me?
Thank you very much.
ziguo
Hi Ziguo , you can see this attach file I hope its useful
https://www.researchgate.net/publication/260131454_BioID_A_Screen_for_Protein-Protein_Interactions
Article BioID: A Screen for Protein-Protein Interactions
Hi Ziguo,
At the present time, no information is available on yeast BioID. But the Roux lab states that some groups are working on it.
http://www.sanfordresearch.org/researchcenters/childrenshealth/rouxlab/bioidresources/
I heard a conference report in October of 2016 of BioID use in yeast from the Kaganovich lab, also the Weissman lab used BIOID in their recent paper. https://www.ncbi.nlm.nih.gov/pubmed/25378625
They have also deposited a bioid plasmid for fusion construction in addgene. Search for BirA.
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