Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View

How to apply Discriminant Analysis suitability, mode to apply and interpretation of results?

Hi, I am in the finishing stage of my thesis (filed - Consumer Behaviour) I have an independent variable (measured with multiple factors) and also a dependent variable (also with multiple...

25 March 2020 2,674 3 View

Referencing with zotero - Is customised version available? Alternatively how to cutomise?

Hello. I am writing my thesis. Recently have started using zotero software for citations. My requirement is APA style - with 1) In-text citation with (author, year) and superscript with number...

21 March 2020 8,925 4 View

How to detect unbinding of a protein from a gold nanoparticle through UV-Vis?

From literature, it could be observed that while binding of gold nanoparticle and protein occurs the UV-VIS peak of gold shifts by a small amount suggesting binding. Although further...

15 October 2018 8,667 3 View

How can I calculate an equivalent dose (in vitro to in vivo)?

15 August 2014 8,174 4 View

Can anyone help me share your detail working protocol for Immunoprecipitation (IP) using "A" or "G" beads?

In the past several years I am doing IP-WB, but so far there is no consistent and standard protocol to work on all IP's. So, here my question is I need very detail and specific steps need to...

14 August 2014 4,515 5 View

Can anyone help me to suggest using 4 colors for Immunofluorescent including nuclear staining?

Currently I am working on one protein with 2 different variants, it is basically nuclear localization, but there is no clue about the 2 variants. If I checked individually means either one may be...

14 August 2014 9,529 4 View

How to generate ROC curve from svm-light output?

My svmlight output for binary classification (+1 for true and -1 for false classes) is like +1 0.001 -1 -0.34 +1 0.9 +1 055 -1 -0.2 I tried generating roc in R using ROCR package. But I...

27 October 2012 4,339 14 View

How to draw ROC curve and RI curve for prediction generation using SVM?

Will Rapidminer tool help me in drawing a ROC curve? Moreover, I tried installing it, but could not.

13 October 2012 6,882 4 View

How to execute cross validation and generate ROC curve in SVMlight?

For doing cross validation is it the parameter x which has to be used? Or is it some other parameter? I am confused. For doing 10 cross validation -x 10 will be fine. Also in SVM light how to...

08 October 2012 5,695 8 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View