Hemalatha Jayabal

4 Questions 1 Answers 0 Followers

Questions related from Hemalatha Jayabal

How to reduce autofluorescence in yeast (Excitation - 488 - 497 nm) ?

I have a fluorescent protein (excitation - 497 nm) expressed on the surface of yeast, however as the cells are auto fluorescent, I am unable to see the expressed and non-expressed populations in...

11 August 2023 8,283 3 View

What concentration of dodecyl maltoside (DDM) detergent to be used for protein extraction from skin tapes?

I understand that the CMC of dodecyl maltoside is approx. 0.17 mM and for effective protein extraction, the detergent concentration is to be higher than that of CMC. 1) I would like to know how...

11 December 2019 4,438 4 View

How to detect unbinding of a protein from a gold nanoparticle through UV-Vis?

From literature, it could be observed that while binding of gold nanoparticle and protein occurs the UV-VIS peak of gold shifts by a small amount suggesting binding. Although further...

16 October 2018 8,768 3 View

Does binding of a protein to a gold nanoparticle change the color of gold nanoparticles?

Binding of a protein to a gold nanoparticle(5-7 nm) increases the size of the complex as validated by a red shift in the uv-vis spectra. Does the overall size increase of AuNP-Protein complex...

31 July 2018 7,868 3 View