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Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

How to do regression analysis in SPSS when the independent variables are manipulated (I only measuring the dependent variables in the survey)?

So in my research the independent variables are manipulated online review content (manipulated valence, platform, etc). There are 6 different groups with different manipulations (conditions). And...

14 July 2019 3,996 3 View

What is the best instrument to check on elements of lubricating additives? Using types of chromatography or any engine test?

To know the elements in PIBSI one of the additives for lubricants

08 May 2019 1,111 3 View

How to do miRNA isolation from hematopoietic stem cell with good purity?

Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According...

14 October 2018 6,123 1 View

Can someone help me with sample size?

Here is the case: comparing systolic blood pressure (in mmHg) in a trial of new drug and placebo. Now I know that the formula of minimum sample size for this case is: 2.((Za + Zb).SD/d)2 Red: 2...

18 January 2017 8,525 5 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

28 July 2016 4,436 3 View

Is no problem if I do Ethanol precipitation with composition glicogen, Na-asetat, before and after phosphorylation PCR product by T4 PNK ?

Dear all, Is there anyone have experience do ethanol precipitation PCR product prior to phosphorylation by T4 polynucleotide kinase? According literature ethanol precipitation by ammonium asetat...

26 July 2016 1,035 3 View

How to design phosphorylation PCR product ?

Dear ALL I want to phosphorylate my PCR product, please give me sugestion how to calculate p mol PCR product.., is there any protocol without converse.., is there any link or literature I can use...

21 July 2016 6,551 1 View

How do I grow single cell become colonies after sorting by FACS in mcf7 cells?

I have already prepared knock out cell by using px459 for knock out mcf7 cell then shorting by using FACS to get single clone, one day after FACS I get around 10 single clone/well ( positive EGFP...

20 December 2015 7,935 6 View

Is there a possibility that the virus accepted by the cells after several days was rejected from the cells, in a stable transfection of a cell line?

I had injected retrovirus containing pBabe.puro.cmv and pcl ampho to the F1 B16 and then after 24 hours and 48 hours I did puromycin selection, The First days and second days the cell decrease...

01 April 2015 3,936 12 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View