Dewi Yuliana

10 Questions 16 Answers 0 Followers

Questions related from Dewi Yuliana

Does ayone know the different between filipin diluted by dmso and diluted by ethanol 100%?

Dear All I have read sigma catalog, filipin can dilute by dmso and ethanol 100%. is there any different function or results filipin staining with two solvent?. please give the suggestion, maybe...

27 April 2017 1,545 2 View

Protokol pilipin staining?

Dear all, I want to try filipin staining at the first time by using filipin sigma (F-9765). please give me suggestion and what kind troubleshooting for filipin staining. best regards, Dewi Yuliana

17 February 2017 6,188 0 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, My products of interest will be approximately around 2300bp in size. In...

29 July 2016 4,602 3 View

Is no problem if I do Ethanol precipitation with composition glicogen, Na-asetat, before and after phosphorylation PCR product by T4 PNK ?

Dear all, Is there anyone have experience do ethanol precipitation PCR product prior to phosphorylation by T4 polynucleotide kinase? According literature ethanol precipitation by ammonium asetat...

27 July 2016 1,179 3 View

How to design phosphorylation PCR product ?

Dear ALL I want to phosphorylate my PCR product, please give me sugestion how to calculate p mol PCR product.., is there any protocol without converse.., is there any link or literature I can use...

22 July 2016 6,711 1 View

What disadvantage using phosphorylation vector (5-OH)?

Dear all, please give information what are disadvantage using dephosphorylation vector (5-OH). what kind literature and theory related about that

21 July 2016 6,716 14 View

Has anyone information about cDNA microarray for Mouse Embryonic Fibroblast (MEF)?

Dear All, Where I can get information about database from cDNA microarray for Mouse Embryonic FIbroblast (MEF)? Thanks very much for your information

18 April 2016 7,034 1 View

Anz advice on Mcf7 cells by limited diution essay?

Dear All, Is anyone has experience get single cells by limited dilution in mcf7 cells?

24 December 2015 2,629 3 View

How do I grow single cell become colonies after sorting by FACS in mcf7 cells?

I have already prepared knock out cell by using px459 for knock out mcf7 cell then shorting by using FACS to get single clone, one day after FACS I get around 10 single clone/well ( positive EGFP...

21 December 2015 8,069 6 View

Is there a possibility that the virus accepted by the cells after several days was rejected from the cells, in a stable transfection of a cell line?

I had injected retrovirus containing pBabe.puro.cmv and pcl ampho to the F1 B16 and then after 24 hours and 48 hours I did puromycin selection, The First days and second days the cell decrease...

02 April 2015 4,064 12 View