Dewi Yuliana

7 Questions 9 Answers 0 Followers

Questions related from Dewi Yuliana

Does ayone know the different between filipin diluted by dmso and diluted by ethanol 100%?

Dear All I have read sigma catalog, filipin can dilute by dmso and ethanol 100%. is there any different function or results filipin staining with two solvent?. please give the suggestion, maybe...

27 April 2017 1,501 2 View

Protokol pilipin staining?

Dear all, I want to try filipin staining at the first time by using filipin sigma (F-9765). please give me suggestion and what kind troubleshooting for filipin staining. best regards, Dewi Yuliana

17 February 2017 6,141 0 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, My products of interest will be approximately around 2300bp in size. In...

29 July 2016 4,514 3 View

Is no problem if I do Ethanol precipitation with composition glicogen, Na-asetat, before and after phosphorylation PCR product by T4 PNK ?

Dear all, Is there anyone have experience do ethanol precipitation PCR product prior to phosphorylation by T4 polynucleotide kinase? According literature ethanol precipitation by ammonium asetat...

27 July 2016 1,105 3 View

How to design phosphorylation PCR product ?

Dear ALL I want to phosphorylate my PCR product, please give me sugestion how to calculate p mol PCR product.., is there any protocol without converse.., is there any link or literature I can use...

22 July 2016 6,632 1 View

How do I grow single cell become colonies after sorting by FACS in mcf7 cells?

I have already prepared knock out cell by using px459 for knock out mcf7 cell then shorting by using FACS to get single clone, one day after FACS I get around 10 single clone/well ( positive EGFP...

21 December 2015 8,001 6 View

Is there a possibility that the virus accepted by the cells after several days was rejected from the cells, in a stable transfection of a cell line?

I had injected retrovirus containing pBabe.puro.cmv and pcl ampho to the F1 B16 and then after 24 hours and 48 hours I did puromycin selection, The First days and second days the cell decrease...

02 April 2015 4,000 12 View