Does anyone use stemcell technologies clonacell TCS medium?

03 November 2014 0 780 Report

I wanted to try clonacell TCS medium rarther than limiting dilution. Does anyone have any experience using it?

Also does anyone know how much methycellulose it contains?

Badges
Science topic

More Trevor Huyton's questions See All

Is society ready to discuss the effects of its slave and colonial past ?

Could Britain's past Colonial and Slave legacy. Be in anyway still having an adverse effect on the mental health and wellbeing of black people. From Education Socially and incarceration.

25 June 2024 4,785 1 View

I have a co-author who has changed her name. Is there an easy way to get that article connected up to her Research Gate profile with her new name?

Any suggestions would be welcome

11 April 2023 8,142 1 View

Does anyone have experience using the Qiagen miRCURY system with other companies primers?

I'm running qPCR on some microRNA samples and I need a new cDNA kit as the MystiCq one was discontinued. I still have all of the MystiCq primers and ready mix, but need a new cDNA kit, I tried...

31 October 2022 5,311 1 View

Where can I find the information of recurrence at the TCGA COAD dataset ?

I downloaded the clinical data of TCGA COAD, but I cannot find the recurrence information. Hope someone can help me on that , thanks

07 October 2022 5,506 0 View

Electrophysiology (in vivo) - agar bridges for reference, has anyone used?

Hello, does anyone have any experience, or know of anyone, that has applied the gold-standard of referencing for ex vivo brain slice recordings to an in vivo setup? If so, could you please pass on...

23 April 2021 346 1 View

Is Fiat Currency Dead as indicated this month's edition of Time Magazine?

Incredible front cover page from Time magazine to coincide with the #nft craze. Time will now accept #cryptocurrency as a form of payment for their digital subscription.

23 March 2021 5,006 4 View

Reference Electrode Comparison for in Vivo electrophysiology... Existing studies/knowledge?

Dear friends and colleagues. Does anyone have any opinions, or know of any studies, regarding the best material (& placement) to use for in vivo electrophyisological Studies? In particular,...

26 February 2021 8,652 0 View

Can you use mixed haploid and diploid datasets for calculation of ABBA-BABA (D) value and f4-ratios?

Hi, I'm looking at the calculations of Malinsky et al (2020), where they detail the logic behind Dsuite's calculations. I have a mixed haploid and diploid species dataset where there is clear...

22 February 2021 9,049 6 View

Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 3,312 3 View

Has anybody used computational linguistics-based, AI-assisted analytics for their research like the covid-19 study below?

https://www.sciencefocus.com/news/covid-19-is-making-its-way-into-our-nightmares/ “The computational linguistics-based, AI-assisted analytics that we used is really a novel approach in dream...

16 November 2020 8,539 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View